氧乐果和吡虫啉对小麦过氧化物酶、谷胱甘肽还原酶及过氧化氢酶活性的影响

仲丽; 吕超; 杨文玲; 梁沛; 史雪岩; 高希武

氧乐果和吡虫啉对小麦过氧化物酶、谷胱甘肽还原酶及过氧化氢酶活性的影响

Effects of imidacloprid and omethoate on the activity of peroxidase, glutathione reductase and catalase in wheat seedlings

摘要

摘要: 为明确常用杀虫剂对小麦抗氧化性的影响,研究了小麦幼苗期用不同浓度氧乐果和吡虫啉的营养液处理后144 h内对其过氧化物酶(POD)、谷胱甘肽还原酶(GR)及过氧化氢酶(CAT)活性的影响。结果表明:用400、800和1 600 mg/L的氧乐果处理小麦幼苗后24 h,POD活性均显著降低;1 600 mg/L的氧乐果处理后6 h,其CAT活性比对照降低了32.9%;各浓度氧乐果处理后144 h,GR活性均显著降低。而用25、50和100 mg/L的吡虫啉处理小麦幼苗后144 h内,只有50 mg/L处理组在12 h时的POD活性比对照升高了65.0%。杀虫剂对小麦幼苗中3种抗氧化酶活性的影响不仅与药剂种类有关,还具有一定的剂量效应与时间效应。

Abstract: In order to examine the effects of insecticides on the antioxidizability of wheat,the activity of peroxidase(POD),glutathione reductase(GR) and catalase(CAT) in wheat seedlings were investigated during 144 h after treatment with imidacloprid and omethoate.The results showed that,comparing with that of the control,the activity of POD in wheat seedlings decreased significantly after being treated with 400,800 and 1 600 mg/L omethoate for 24 h respectively,and the activity of CAT decreased 32.9% after being treated with 1 600 mg/L omethoate for 6 h.After being treated by 400,800 and 1 600 mg/L omethoate for 144 h,the activity of GR in wheat seedlings decreased significantly.When wheat seedlings were treated with 25,50 and 100 mg/L imidacloprid respectively,only the activity of POD of wheat seedlings increased 65.0% after being treated with 50 mg/L imidacloprid for 12 h comparing with that of the control.The results showed that activity of POD,GR and CAT in wheat seedlings were influenced by the types of insecticides,as well as the concentration of insecticides and treatment time of insecticides.

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