超高效液相色谱-串联质谱法测定香蕉中壬基酚聚氧乙烯醚及其降解产物

Determination of nonylphenol polyethoxylates and its metabolite nonylphenol in banana by UPLC-MS/MS

摘要: 为了研究壬基酚聚氧乙烯醚在食品中的残留量及安全性,通过乙腈提取、固相萃取法净化,建立了香蕉中壬基酚聚氧乙烯醚(NPnEO)及其降解产物壬基酚(NP)的超高效液相色谱-串联质谱(UPLC-MS/MS)测定方法,并对流动相、监测离子和校正曲线等进行了优化和探讨。结果表明:在5~40 μ g/kg添加水平范围内,NPnEO及NP的添加回收率在75%~117%之间,相对标准偏差(RSD)在3.2%~9.6%之间;NPnEO的检出限在0.005~0.04 μ g/kg之间,NP的检出限(LOD)为0.5 μ g/kg;该方法快速、灵敏,准确度高,符合残留检测的要求。

Abstract: In order to study the residues and safety of pesticide adjuvant nonylphenol ethoxylate(NPnEO)in food,a quick and sensitive analytical method for nonylphenol ethoxylate and its metabolite nonylphenol in banana was established with UPLC-MS/MS.The samples were extracted by acetonitrile and cleaned by solid phase extraction column.Several UPLC-MS/MS conditions that included the mobilephase,monitor ions and the selection of calibration of the measurement were studied.The recovery ranged from 75% to 117% at three spiking levels from 5 -40 μ g/kg with relative standard deviations of 3.2%-9.6% ,meanwhile,the limit of detection of nonylphenol ethoxylate were 0.005-0.04 μ g/kg and nonylphenol was 0.5 μ g/kg.The method was sensitive and rapid.