杠柳杀虫活性成分的分离

Isolation of the insecticidal ingredients from Periploca sepium

摘要: 采用柱层析分离、高效液相色谱切分和生物活性追踪法,从杠柳 Periploca sepium 根皮甲醇提取物中分离出2个具有杀虫活性的化合物(G1和G2),经鉴定其分别为已知物杠柳新苷D和F。生物活性测定结果表明,化合物G1和G2 对3龄粘虫 Mythimna separata 48 h的胃毒致死中浓度(LC50)分别为0.39和0.34 mg/mL, 对小菜蛾 Plutella xyllostella 48 h的胃毒LC50值分别为1.21和1.39 mg/mL。

Abstract: Two compounds,periplocoside D and F were isolated from the methanol extract of the root barks of Periploca sepium by silica gel chromatograph,high pressure liquid chromatograph(HPLC) and bioassay-guided fractionation.The bioassay results showed that periplocoside D and F had stomach toxic activity with the LC50 value of 48 h 0.39 and 0.34 mg/mL against 3rd instar larvae of Mythimna separata,and against 3rd instar larvae of Plutella xyllostella with the LC50 value of 48 h 1.21 and 1.39 mg/mL.