Abstract: The effect of environmental factors such as temperature,relative humidity(RH),water quantity,photoperiod,illuminance and leaf on the results of laboratory bioassay of spinosad to Frankliniella occidentalis(Pergande)was studied using method of Munger cell.The results showed that during the bioassay conditions ranges of temperature 25-30 ℃, RH 50% -70%,water quantity 0.5-1.5 mL,photoperiod 16 h:8 h-24 h:0 h(L:D),illuminance 6 500-13 000 lx and with the leaves of Rosa chinensis,Cucurbita pepo,Raphanus sativus,Phaseolus vulgaris,Malus pumila mill,Apiumgraveolens,Spinacia oleracea,the laboratory bioassay results preserve stationary.The leaf cilium density has negative correlation with the bioassay result of spinosad to F.occidentalis,and the related coefficient was 0.919;the length of leaf cilium has no correlation with the bioassay result.