利用分子印迹固相萃取技术净化韭菜中残留的敌百虫

Cleanup of trichlorfon extract from leeks by molecularly imprinted solid phase extraction technique

摘要: 以敌百虫为模板,以 α -甲基丙烯酸(MAA)为单体、偶氮二异庚腈(ABVN)为引发剂、三羟甲基丙烷三甲基丙烯酸酯(TRIM)为交联剂以及乙腈为溶剂,在 n (敌百虫) : n (MAA) : n (TRIM)=1 :8 :40的条件下制备了敌百虫的分子印迹聚合物(敌百虫-MIP)。该MIP对敌百虫的吸附率是非印迹聚合物(NIP)的2.4倍,印迹效果良好。敌百虫-MIP-固相萃取柱对韭菜样品中杂质的去除效果明显好于C18固相萃取柱。在1 ~0.05 mg/kg添加水平内,韭菜中敌百虫的添加回收率在92.7% ~84.7%之间,相对标准偏差( RSD )在8.5% ~4.3%之间,检测限(LOD)为0.01 mg/kg。

Abstract: Molecularly-Imprinted Polymer (MIP) for trichlorfon were prepared by using methacrylic acid (MAA) as the functional monomer,2,2'-azobis-(2,4-dimethylvaleronitrile) (ABVN) as initiator,trimethylolpropane trimethylacrylate (TRIM) as cross-linker,acetonitrile as solvent.The ratio of n (trichlorfon) : n (MAA) : n (TRIM) was 1 :8 :40.Solid phase extraction (SPE) column packed with the MIP show superior clean-up effect than normal SPE column for leek sample.Within 1.0-0.05 mg/kg spiked level,the recovery of trichlorfon from leek sample was ranged from 92.7% to 84.7% with RSD of 8.5%-4.3%.Limit of detection (LOD) of 0.01 mg/kg was achieved.