抗菌蛋白磷酸盐结合蛋白前体的分离及其产生菌的鉴定

Isolation of antifungal protein phosphate-binding protein precursor and identification of its producing strain

摘要: 以抑菌活性生物测定结果为指导,采用硫酸铵沉淀、离子交换柱层析、凝胶柱层析和SDS-聚丙烯酰胺凝胶电泳等方法,从采自广西土壤的1株放线菌菌株GXWl发酵液中分离到1种未见文献报道具有抑菌活性的蛋白,经质谱鉴定并与NCBInr蛋白数据库比对,认为其应为磷酸盐结合蛋白前体 (phosphate-binding protein precursor,pre-PBP),分子质量为39.18 ku。根据其形态特征、培养特征、生理生化特性及16S rDNA序列分析,将菌株GXWl归入链霉菌属,并鉴定为锈赤蜡黄链霉菌Streptomyces rubiginosohelvolus。

Abstract: Guided by the results of the bioassay against Bacillus cereus,a protein that the antimicrobial activity was not reported in the literature was isolated from the fermentation broth of actinomycete GXWl which was collected from the soil of Guangxi province by ammonium sulfate precipitation,ion-exchange chromatography,gel column chromatography and SDS-PAGE in turn.According to the analysis of protein mass spectroscopy by a BLASTn search in NCBInr database,the antifungal protein was identified as phosphate-binding protein precursor with the molecular weight of 39.18 ku.The strain GXWl was characterized as Streptomyces rubiginosohelvolus based on the morphological characteristics,cultural characteristics,physiological and biochemical characteristics,as well as phylogenetic analysis of 16S rDNA sequence.