分散固相萃取-液相色谱-串联质谱法检测香蕉和葡萄中苯锈啶残留

Dispersive solid phase extraction and liquid chromatography-tandem mass spectrometry for the analysis of fenpropidin residue in banana and grape

摘要: 采用液相色谱-串联质谱仪(LC-MS/MS)建立了香蕉和葡萄中苯锈啶残留的分析方法。样品采用乙腈振荡提取,N-丙基乙二胺(PSA)分散固相萃取净化,ESI(+)电离方式,在多反应监测模式(MRM)下选择m/z 274.3→147.1为定量离子对进行检测。结果表明,在香蕉和葡萄基质中苯锈啶的平均回收率在94.1%～118.2%之间,相对标准偏差在1.6%～8.8%之间,方法定量限(LOQ)为0.01 mg/kg。该方法操作简便,适用于苯锈啶主要登记作物中的残留检测。

Abstract: A method for the determination of fenpropidin residue in banana and grape was developed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).The sample was extracted with acetonitrile and further cleaned up by dispersive solid phase extraction utilizing primary secondary amine (PSA).The mass spectrometer with interface of electro-spray ionization in positive ion mode (ESI+) was operated in multi-reaction monitoring (MRM) mode.The ion transition (m/z 274.3→147.1) was monitored for quantification.Average fortified recoveries ranged from 94.1%-118.2% with relative standard deviations 1.6%-8.8% in banana and grape samples.Limit of quantifications (LOQs) were 0.01 mg/kg.The method was convenient and suitable for determination of fenpropidin residues in various commodities on which fenpropidin were applied.