气相色谱法检测辣椒中7种拟除虫菊酯类农药残留

Determination of 7 pyrethroid residues in pepper by gas chromatography

摘要: 建立并优化了新鲜辣椒中甲氰菊酯、高效氯氟氰菊酯、氯菊酯、氟氯氰菊酯、氯氰菊酯、氰戊菊酯、溴氰菊酯7种拟除虫菊酯类农药残留的气相色谱(GC-μECD)分析方法。采用50 mL V(乙腈):V(水)=3:1的混合溶剂提取,二氯甲烷萃取, N-正丙基乙二胺(PSA)净化,HP-1毛细管色谱柱分离,气相色谱-电子捕获检测器(GC-μECD)测定。在添加水平为0.017~0.64 mg/kg下的平均回收率为74.0%~105.6%,相对标准偏差为3.3%~15%。该方法准确、灵敏,可同时测定辣椒中7种拟除虫菊酯类农药的残留量。

Abstract: The residue determination method of seven pyrethroids (fenpropathrin,lambda-cyhalothrin,permethrin,cyfluthrin,cypermethrin,fenvalerate,deltamethrin) in pepper was established and simplified by using gas chromatography (GC-μECD) analysis.Samples were extracted with 50 mL V(acetonitrile):V(water)=3:1,the extracting solution was purified with dichloromethane and N-(n-propyl)ethylenediamine(cleanert PSA),separated by using HP-1 capillary column and determined by using the gas chromatography-electron capture device.The average recovery was 74.0%-105.6% and the relative standard deviation was 3.3%-15% at spiked level of 0.017-0.64 mg/kg.The result showed that the method was accurate and sensitive.It could determine seven pyrethroid residues in peppers simultaneously.