二氯喹啉酸降解菌的分离鉴定及降解特性分析

Isolation,identification and characterization of an Alcaligenes strain capable of degrading quinclorac

摘要: 采用富集培养技术从长期施用二氯喹啉酸的土壤中分离得到1株能够降解二氯喹啉酸的细菌,将其命名为J3,通过生理生化特性和16s rDNA同源性序列分析,鉴定其为产碱菌属(Alcaligenes sp.)。在二氯喹啉酸初始质量浓度为100 mg/L、接种量为4%、pH 7、30 ℃条件下,接种后第6天,菌株J3对二氯喹啉酸的降解率可达到70%以上;在25 ℃、接种量为4%条件下,其生物修复作用可使受二氯喹啉酸(25%田间推荐剂量)药害盆栽烟草的恢复率达到69%。

Abstract: A bacterium strain designated as J3,able to degrade quinclorac efficiently,was isolated from soil with long-term application of quinclorac by enrichment culture method.Base on biochemical-physiological identification and sequence analysis of the 16S rDNA,strain J3 was identified as Alcaligenes sp.. At initial quinclorac concentration of 100 mg/L and J3 inoculation quantity of 4%,more than 70% of quinclorac was degraded in 6 days at pH 7 and temperature of 30 ℃.At J3 inoculation quantity of 4%,temperature of 25 ℃,quinclorac concentration of 25% of its recommendation dose,the rehabilitation rate of the potted tobacco reached 69%.