Isolation and purification of madumycin II from fermented broth of Xenorhabdus bovienii SN269

Compound D3 was isolated from fermented broth of Xenorhabdus bovienii SN269 by silica gel column chromatography and Sephadex chromatography. The structure of D3 was identified as madumycin Ⅱ, by comparing the 1H NMR and 13C NMR, ESI-MS and specific rotation data with the corresponding data reported in published literatures. The antifungal activity of madumycin Ⅱ against phytopathogenic fungi was determined by mycelium growth rate method. The results showed madumycin Ⅱ could effectively inhibit the mycelium growth of Phytophthoa capsici and Botrytis cinerea, and the EC50 values were 35.32 mg/L and 35.40 mg/L, respectively.