Residues of 21 fungicides in Fragaria ananassa by modified QuEChERS and gas chromatography-tandem mass spectrometry

In order to determine 21 fungicides (spiroxamine, metalaxyl, tetraconazole, nitrothal-isopropyl, cyprodinil, penconazole, fluopyram, triflumizole, mepanipyrim, E-metominostrobin, imazalil, myclobutanil, kresoxim-methyl, cyflufenamid, trifloxystrobin, propiconazol, fenhexamid, epoxiconazole, fenamidone, boscalid, azoxystrobin) in Fragaria ananassa, the sample preparation method and GC-MS/MS analysis parameters were optimized. The sample was first extracted by acetonitrile. During the DSPE procedure, PSA and GCB were used as sorbents. After concentrated, the extract was dissolved by a mixed solvent V (hexane):V (acetone)=9:1. The extract was then determined with GC-MS/MS. Linear relationships between peak area and mass concentration of 21 fungicides were obtained in the range of 0.005-0.2 mg/kg. When the spiked levels were 0.01, 0.05 and 0.1mg/kg, the average recoveries all ranged from 70 to 122% with RSD < 20% (n=5). This method is suitable for the rapid and accurate detection of 21 fungicides in F. ananassa.