The rapid detection method for resistance to five neonicotinoid insecticides and sulfoxaflor in Myzus persicae

Bioassay method of residual film in glass tube was employed to determinate the LC90 values of Myzus persicae susceptible strain (SN) to six insecticides, including imidacloprid, acetamiprid, thiamethoxam, clothianidin, nitenpyram and sulfoxaflor. The LC90 values were then used as the diagnose doses for the detection of the sensitivity variations of M.persicae field populations to the six insecticides. Furthermore, the correlation analysis between the mortalities of M.persicae field populations under the diagnose doses of those six insecticides and the resistance levels obtained by leaf dipping bioassay were conducted. The accuracy of the tested resistance levels of M.persicae field populations to the six insecticides using the diagnose doses were verified. The results showed that the LC90 of SN strain to imidacloprid, acetamiprid, thiamethoxam, clothianidin, nitenpyram and sulfoxaflor, were 150.01, 1 170.81, 54.19, 951.34, 245.98 and 133.60 ng/cm2, respectively. Under the treatments of diagnose doses of the six insecticides, the mortality of field population from Zhumadian region of Henan Province (ZM) was between 82% and 96%, the mortality of field population collected from cabbage in Yutian region of Hebei Province (GL) was between 35% and 82%, and the mortality of population collected from peach in the same region (TS) was between 3% and 30%. The correlation analysis showed that the mortalities of M.persicae field populations under the established insecticides diagnose doses were negative correlated with their resistance levels to the six insecticides, with the correlation coefficient between 0.818 8 and 0.999 9. Meanwhile, the theoretical resistance levels of field population from Nanjing region of Jiangsu Province (NJ) to the six insecticides calculated by the correlation equations were close to the actual detected resistance levels. Therefore, through the bioassay method of residual film in glass tube, the LC90 values of the six insecticides to SN strain were established as the diagnose doses. By examining the mortalities of field populations of M.persicae under the diagnose doses, the sensitivity variations of M.persicae field populations to the six insecticides could be rapidly characterized. Moreover, a preliminary assessment of the resistance levels was conducted.