Development and application of a screening method for four kinds of triazine herbicides determination in wine samples using SA-DLLME-SFO-HPLC

A new method, using on surfactant assisted dispersive liquid-liquid microextraction based on solidification of floating organic droplets (SA-DLLME-SFO), and high performance liquid chromatography (HPLC) has been developed for the enrichment and determination of triazine herbicides in wine samples. Various experimental parameters affecting the extraction efficiencies, such as extraction solvent, extraction solvent volume, surfactant volume, extraction time and salt concentration were investigated and optimized. The following optimized conditions:extraction solvent, 20 μL1-undecanol; dispersive solvent, 2 μL 0.25 g/L Tween-20; extraction time, 10 min; NaCl addition, 250 g/L. Under the optimized conditions, the linear ranges for simazine, atrazine, terbuthylazine and prometryn were from 0.008 to 5 mg/L. The detection limits obtained for simazine, atrazine, terbuthylazine and prometryn were 2.4, 1.8, 2.0 and 2.8 μg/L, respectively. The enrichment factor were between 24.5 and 32.4. The recoveries of the different compounds were in the range of 84%-102% with the relative standard deviations were between 2.1% and 6.9% (n=5) at the spiked levels of 0.008, 0.08 and 2.4 mg/L. The proposed method was successfully applied to the analysis of simazine, atrazine, terbuthylazine and prometryn in wine.