Dualcontrolplasmidconstructionandexpressionoptimizationof

Sorghumbicolor

ARSforanovelsorgoleoneherbicidebiosynthesis

Allelochemicals offer great potential for the development of future herbicides. Sorgoleone is a kind of allelochemicals secreted by Sorghum bicolor. It can significantly inhibit the growth of many weeds. S. bicolor alkylresorcinol synthase (Sb_ARS) is a key regulatory enzyme in the pathway of sorgoleone biosynthesis. When Sb_ARS is expressed intracellularly using Escherichia coli as the host, the disruption of cell membrane can be time-consuming and costly. In this study, Sb_ARS gene was optimized, chemically synthesized and expressed in a heat-inducible autolytic E. coli BL21(DE3) system by a novel dual control expression plasmid. After optimizing IPTG/heat-induced expression conditions, Sb_ARS activity was found to be 160.04% ± 8.12%, and the lysis efficiency was improved to 82.81% ± 3.14%. These results would lay a foundation for the biosynthesis of new herbicide sorghum.