IdentificationofabiocontrolactinomycetestrainCY-14andtheeffectofitsfermentationbrothon<italic>Colletotrichummusae</italic>

Banana anthracnose is caused by Colletotrichum musae. In China, it is one of the main diseases that happens during the post harvest storage, including transportation and sale, which seriously affects the north transportation and export of the banana. A actinomycete strain of CY-14, which had antagonistic effect on C. musae, was isolated from the soil. The strain was preliminarily identified by morphology, physiological and biochemical characteristics, and 16S rDNA sequence analysis. The bioactivity of its fermentation broth was also evaluated. The results showed that strain CY-14 was preliminarily identified as Streptomyces sp. The inhibition rate of 10 times diluted fermentation broth to C . musae was 92.5%. The fermentation broth also had a broad-spectrum antifungal activity to other 8 kinds of plant pathogenic fungi. In addition, the fermentation broth had good thermal stability. After heated up at 100 ℃ for 2 hours, the inhibition rate of 10% fermentation broth was 55.7%. The 1 000 times diluted fermentation broth could completely inhibited the spore germination of C . musae. While the pure fermentation broth had the same control effect as 1 000 mg/L chlorothalonil. Strain CY-14, as a biocontrol actinomycetes of banana anthracnose, has a potential application in the discovery of new antibiotics or active compounds, and is well worthy of further study.