EffectsofimidaclopridorsulfoxaflorontheexpressionofcytochromeP450genesingreenbug(<italic>Schizaphisgraminum</italic>Rondan)basedonRNAsequencing

To identify the cytochrome P450 (P450) genes involved in the metabolic regulation of imidacloprid or sulfoxaflor, the greenbug was treated with imidacloprid or sulfoxaflor for 24 h using the dipping method. Then the differentially expressed P450 genes were identified through transcriptome sequencing and quantitative real-time PCR (qPCR). The results indicated that the expression of 19 P450 genes were up-regulated and 15 P450 genes were down-regulated in the greenbug treated with imidacloprid. In terms of the greenbug treated by sulfoxaflor, the expression of 18 P450 genes were up-regulated and 15 P450 genes were down-regulated. Furthermore, 12 P450 genes affected by imidacloprid were validated by the qPCR method, and the results demonstrated that the expression levels of these genes were consistent with that of the DGE library. These results provide a basis for further study of the detoxification of imidacloprid and sulfoxaflor by P450s in the greenbug.