Enantioseparation and on-column preconcentrstion of imazalil by capillary electrophoresis

The enantioseparation of imazalil was conducted by capillary electrophoresis, and the effects of chiral selector β-cyclodextrin(β-CD), organic additives, NaH₂PO₄, NH₄H₂PO₄ and separation voltage on the resolution were studied. And the effects of the injection pressure and preconcentration times on the on-column preconcentration of imazalil were studied by the stacking method. The results showed that the best resolution was achieved under the following conditions: separation voltage 20 kV, β-CD 5 mmol/L and a buffer system of 2.5 % isopropanol + 75 mmol/L NaH₂PO₄ + 5 mmol/L NH₄H₂PO₄ . And the resolution was up to 3.0. The highest enrichment factor was obtained at the separation voltage of 20 kV using 5 mmol/L β-CD, a buffer system of 75 mmol/L NaH₂PO₄ + 5 mmol/L NH₄H₂PO₄ + 50 mmol/L H₃PO₄, and the injection conditions of 13.8 kPa×99.9 s. And the enrichment factor was up to 91-92 times.