Simultaneous determination of MCPA-isooctyl and its metabolite MCPA in maize by ultra-performance liquid chromatography-tandem mass spectrometry
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Graphical Abstract
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Abstract
An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the simultaneous determination of MCPA-isooctyl and its metabolite MCPA residues in maize (fresh maize, maize kernel and maize stover). The samples were acidified with hydrochloric acid, extracted with acetonitrile, cleaned-up by NH2-SPE column, analyzed by UPLC-MS/MS in multiple reaction monitoring (MRM) mode, and quantified by external standard method. The results showed that the average recoveries of MCPA-isooctyl and MCPA were in the range of 82%~107% at the spiked levels of 0.01~0.5 mg/kg with the relative standard deviations (RSDs) of 0.81%~8.9%. The limits of detection (LOD) of the method were 0.38~1.42 μg/kg and the limits of quantification (LOQ) were 1.28~4.76 μg/kg. The method is reliable and sensitive, and meets the requirements of pesticide residue analysis, and it can achieve the simultaneous detection of MCPA-isooctyl and its metabolite MCPA in corn.
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