WU Xiaopeng, XU Xiao, WENG Liangna, YANG Yafang, XU Zhi. Determination of seventeen plant growth regulator residues in four tropical fruits by a modified QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Pesticide Science, 2024, 26(6): 1171-1180. DOI: 10.16801/j.issn.1008-7303.2024.0106
    Citation: WU Xiaopeng, XU Xiao, WENG Liangna, YANG Yafang, XU Zhi. Determination of seventeen plant growth regulator residues in four tropical fruits by a modified QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Pesticide Science, 2024, 26(6): 1171-1180. DOI: 10.16801/j.issn.1008-7303.2024.0106

    Determination of seventeen plant growth regulator residues in four tropical fruits by a modified QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry

    • A method based on ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) technology was developed for the rapid and simultaneous detection of 17 plant growth regulators (PGRs) in the fruits of carambola (Averrhoa carambola), papaya (Carica papaya), avocado (Persea americana), and wampee (Clausena lansium). The quick, easy, cheap, effective, rugged, and safe (QuEChERS) method was adopted for the pre-treatment of fruit samples in this study, and the pre-treatment factors, such as the phase-out salt, extraction method, extraction solvent, extraction time, and purification materials were optimized. The final pre-treatment method was determined to use 25 mL of acetonitrile for the extraction of 10 g samples by votexing for 10 min, followed by the phasing-out with the mixed salts including 4 g MgSO4 + 1 g NaCl + 1 g Na3Cit·2H2O + 0.5 g Na2HCit·1.5H2O , Then 150 mg of MgSO4 and 30 mg of C18 was used as purification materials to purify the extracts. The 17 target PGRs were detected using multi-reaction monitoring (sMRM) mode and quantified with the matrix-matched external standard method. The method was validated according to the European guideline of SANTE/11312/2021(V2). The linearity for the 17 PGRs was excellent in the range of 0.05 to 20 μg/kg or 0.5 to 20 μg/kg, with correlation coefficients all greater than 0.999. The results at low, medium, and high spiked levels showed that the intra-day and inter-day recovery rates were 80% to 120% with relative standard deviations all below 20%. The results confirmed that the proposed method was suitable for the routine detection of these pesticides in the four fruit samples. The established method was used to detect the fruit samples purchased from the local markets in Haikou city, China. The residues of 13 PGRs were detected, among which trinexapac-ethyl and 5-nitroguaiacol sodium salt were detected in the samples of all four fruits. This study provides technical reference for the establishment of multi residue analysis methods for PGRs in tropical fruits.
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