Simultaneous determination of fluopyram,trifloxystrobin and its metabolite trifloxystrobin acid in fruits by gas chromatography

A method was established for determining fluopyram,trifloxystrobin and its metabolite trifloxystrobin acid residued in fruits by gas chromatography(GC).Residues were extracted with acetonitrile,salted-out by sodium chloride and magnesium sulfate,cleaned up by primary secondary amine (PSA),and determined by GC equipped by ECD.The results showed that the method was validated using fruits samples spiked with pesticides at concentration levels ranging from 0.05 to 1 mg/kg.The average recovery is from 79% to 120%,with relation standard deviations between 0.7% to 16%.The limits of detection (LOD) were 0.01,0.015 and 0.01 mg/kg for fluuopyram,trifloxystrobin and trifloxystrobin acid in the different fruits samples.And the limit of quantification (LOQ) was 0.05 mg/kg. The method is rapid,easy and stable for determination of fluopyram,trifloxystrobin and trifloxystrobin acid in fruits.