Chiral Separation of Quizalofop on Amylose-Chiral Stationary Phase by HPLC

Amylose se-tris(3,5-2 meth phenyl carbamate) was synthesized and used as the chiral stationary phase upon adsorption over silica gel. This stationary phase offered a practically useful chiral column with a high enantiomer separability. Amylose was allowed to react with an excess of 3,5-2 meth phenyl carbamate in pyridine for 48h. The obtained carbamate was dissolved and adsorbed on silica gel which has been treated with 3-aminopropyltriethoxysilane. The weight ratio of the carbamate to the silica gel was 0.45/2.55. The quizalofop was resoluted on amylose tris(3,5 dimethphenylcarbamate) chiral stationary phase. Separation was carried out with a hexane/2 propanol mixture at a flow rate of 0.5 mL·min-1 at room temperature. The effects of 2-propanol concentration on the retention and resolution had been investigated