Study on Resistance Selection and Activity of Detoxifieation Enzyme in Tetranychus cinnabarinus (Boiduval)

Fenpropathrin and abamectin were used to select the resistance in Tetranychus cinnabarinus in laboratory. After 42 and 40 generations selection with abamectin and fenpropathrin, Tetranychus cinnabarinus developed 8.7 and 68.5-fold resistance to the two acaricides respectively. The major resistant mechanism to abamectin was the increasing of the activity of carboxylesterases (CarE), glutathion S-transferase (GSTs) and mixed function oxidase (MFO), and the activity in resistant strain developed 2.7, 3.4 and 1.4-fold contrasted to susceptible strain respectively. The activity of GSTs in the strain selected with fenpropathrin developed 2.8 fold contrast to susceptible strain, which meant the resistance was related with the increasing of the activity of GSTs in resistant strain. The result of the kinetic determination of CarE showed that the structure of CarE in the strain selected with abamectin has been changed.