Residue Analysis Method of Flusilazole in Grape and Soil by HPLC

A method was developed to determine residue of flusilazole in grape and soil by HPLC. The lowest detection limit of instrument for flusilazole was 3.0×10-11 g. The limit of quantitation was 0.01 mg/kg.The average recovery and coefficient of variation of this method were 92.6%~99.0% and 1.7%~13.3%,respectively. HPLC system consisted of 15 cm×4.6 mm (id) Shim-pack VP ODS HPLC column equipped with a 3 cm ODS guard column. Chromatographic conditions as follows: mobile phase, acetonitrile+water(45∶55,V/V); flow rate, 1 mL/min; column temperature,45℃;detection wavelengths, 195 nm.