Hapten Design and Antibodies Development of Milbemycin Oxime

To produce antigens susceptible to raise antibodies for milbemycin oxime A4、A3(MILO),the 5-O-succinoylmilbemycin-oxime were bound to bovine serum albumin (BSA) by the carbodiimide activation. Two New Zealand white rabbits were immunized with MILO-BSA and the titers of antiserum had reached 1∶ 12 800. A MILO-OVA-based CI-ELISA was conducted with the antiserum, the response range for milbemycin oxime was between 16.00 ~4 394.18 ng/mL and the limit of detection was 6.28 ng/mL.