The Separation,Purification and Bioassay of the Effective Metabolites of SPRI-231

For the screening of new agro-antibiotics, a strain of streptomycetes was isolated from the soil sample collected in Guangxi Province and named SPRI-231. The strain's active metabolites of the strain were isolated and purified with solvent extraction, silica gel chromatography, TLC, HPLC etc. The pure components of compound SPRI-231 (Ⅰ) and SPRI-231 (Ⅱ) were obtained. Based on physico-chemical properties, as well as UV、IR、ESI-MS、ESI-HRMS and elemental analysis, their molecular formula were confirmed as C51 H91 N3 O21 and C52 H93 N3 O21 ,respectively. These two compounds have good effect in protecting plant fungi diseases, the inhibition rate of refined extracts of SPRI-231(Ⅱ) at 500 mg/L against cucumber Botrytis cinerea and cucumber Colletotrichum lagenarium were 83.02% and 90.40%,respectively.