Enantioresolution and Study of Thermodynamical Property of Napropamide by High Performance Liquid Chromatography

The enantiomer of napropamide was resolved under reverse phase high performance liquid chromatography(HPLC) using Chiralcel(AD-RH) and Chiralcel OD-RH with acetonitrile-water as a mobile phase.The effects of the content of acetonitrile in mobile phase,flow-rate and temperature on the chiral separation of napropamide were studied.The chiral separation mechanism of napropamide enantiomer on the CSPs was analysed by thermodynamical parameters.The results showed that the △△H0 of ADRH and OD-RH chiral column were-1 160.79 J/mol and-673.40 J/mol respectively,namely the stereoselectivity of Chiralcel AD-RH was better than Chiralcel OD-RH and good enantiomeric separations were achieved with both CSPs under the experiment of flow-rate 0.5 mL/min,acetonitrile-water 50 ∶50 in volume and temperature 10～35℃.