Synthesis of Artificial Antigen and Preparation ofPolyclonal Antibodies against Cyhalothrin and it's Metabolites

Two types of haptens,the carboxylated propyl amino derivative of the acid moiety (HCA1) that is the hydrolyzed product of cyhalothrin,and the carboxylated ethyl carbonyl derivative of the alcohol moiety (HCB2) were synthesized.HCA1 and HCB2 were conjugated with the carrier proteins bovine serum albumin (BSA) by the active ester method to obtain the artificial immunogens.HCA1、HCAS and HCB2 were conjugated with ovalbumin (OVA) by the mixed anhydride method to obtain the coating antigens respectively.The polyclonal antibodies (PAbs) were obtained after the immunogens immunized to the New Zealand rabbits.One PAbs,which raised against the former hapten HCA1,was reactive with cyhalothrin and cyhalothric acid (IC50:33.12 mg/L,0.95 mg/L).However,the other PAbs,which raised against the later hapten HCB2,was not particularly reactive with cyhalothrin.