A Quick-test for Detection of Herbicide Resistance to Tribenuron-methyl in Galium aparine

Ten biotypes of Galium aparine were sampled from Shandong,Henan,Anhui and Shaanxi Province.A single concentration discrimination method in Petri dish for detection of Galium aparine resistance to tribenuron-methyl was established and validated by greenhouse pot tests and Petri dish test.The results showed that a significant difference of germination rate between resistance and susceptible biotypes was obtained under the discrimination concentration,85 mg (a.i.)/L of tribenuron-methyl.The resistance level tested by the Petri dish bioassay and greenhouse pot tests are in accordance with those of the single concentration discrimination method. The resistance of all the potential resistant biotypes to tribenuron-methyl,except those from Tai'an,Shandong Province,and Zhouzhi,Shaanxi Province was developed.The results suggested that this single concentration discrimination method is reliable and feasible.