Enantioseparation of Novel α-Aminophosphonate Esters on the Amylose Derivative Chiral Stationary Phases by HPLC

The direct HPLC enantioseparation of six O,O'-dialkyl-α-(fluorinated phenylethy1)amino-3,4,5-trimethoxybenzy1 methy1 phosphonates on coated and immobilized amylose tris-(3,5-dimethyphenylcarbamate) chiral stationary phases (Chiralpak AD-H and Chiralpak IA) was presented.In n-hexane/2-isopropanol and n-hexane/ethanol mixtures,all studied compounds can be baseline separated on AD-H column,AD-H column exhibits better separation effect for all compounds than IA column.The effect of temperature and ethanol organic modifier concentration on the resolution was investigated on AD-H column.The results showed in the range of 10 ~40℃,resolution factors and sepatation factors increased with decreasing temperature; in the range of 5% ~15% ethanol in n-hexane,resolution factors increase with decreasing solvent polarity and separation factors were relatively stable.AD-H was recommended to carry out chiral semi-preparative separation of all compounds.