Analysis of Imidacloprid and Acetamiprid Residue in WheatSeeds by Matrix Solid-Phase Dispersion and HighPerformance Liquid Chromatography

Analytical method using matrix solid-phase dispersion (MSPD) clean-up for the analysis of imidacloprid and acetamiprid in wheat seeds was developed.Wheat seed sample was mixed in a glass mortar with a bonded-phase solid support material.Then the solid support and sample were manually blended together using a glass pestle.The blended material was packed into a column containing activated C18 and eluted with acetonitrile.The eluate was concentrated and analyzed by high-performance liquid chromatography with ultraviolet detection.The linearity of response ranges of imidacloprid and acetamiprid was obtained from 0.05 to 0.8 mg/L and 0.1 to 1.0 mg/L, the correlation coefficient were 0.999 6 and 0.999 7, the recoveries ranged from 88.5% to 98.4% and from 93.2% to 98.9% respectively.At the fortified levels of 0.05, 0.1 and 0.5 μg/g, the relative standard deviations ranged from 2.0% to 3.8% and from 3.6% to 9.8%, the limit of detection were 0.1 ng and 0.4 ng,the limit of quantification were 0.04 mg/kg and 0.1 mg/kg,respectively.