Isolation and Identification of the Antibacterial Composition from theFermentation Broth of Endophytes in Celastrus angulatus (Strain Hd3)

Five antibacterial compounds were isolated from the fermentation broth of endophytes in Celastrus angulatus (strain Hd3) through silica gel columns and HPLC by bioassay-guided fractionation.These chemicals were elucidated as 3'-chlorotrypacidin,asterric acid, methyl asterrate,methyl dichloroasterrate and methyl chloroasterrate by NMR and MS.The bioassay results showed that the inhibition rates of 5 compounds against spore germination of Curvularia lunata were 100％,67.6％,17.3％,29.1％,30.4％ at the concentration of 500 mg/L, respectively;and 3'-chlorotrypacidin and asterric acid showed strong antibacterial activity on Bacillus subtilis.