Study on the Determination of Triptolide and Total Alkaloids inTripterygium wilfordii Hook. f.Cultures

The content of triptolide and the total alkaloids in the root bark,leaves,the cultures and the nutrient solution were determined based on the determination of triptolide by HPLC and UV-spectroscopy method for total alkaloids in the same extract.Samples were extracted with ethyl acetate and cleaned up on an aluminum column,followed by dissolution in 45% methanol solution.The results showed that the linear relationship of the triptolide concentrations and peak areas was in the range of 1~100 μg/mL (R2=0.999 9).The linear relationship of the total alkaloids concentrations and absorbance was good in range of 5~100 μg/mL (R2=0.999 8).In the same extract the average recovery of triptolide was 100.5% (RSD=0.63%)and the average recovery of total alkaloids was 99.8% (RSD=1.22%).It's found that the content of triptolide in the root callus was about 1.25 times as that in the root bark.The yield of triptolide was 56% and that of the total alkaloids was 65% after filtering adventitious roots in the culture medium.The triptolide yield of 1 g per adventitious root was about 10 times that of root bark.The total alkaloids yield of 1 g per adventitious root was about 2 times that of root bark.