Determination of Seven Fluoroquinolones Residue in AquaticProducts by Solid-phase Extraction-HPLC

A reversed-phase HPLC method for analyzing seven fluoroquinolones residue in aquatic products (Anguilla anguilla,Penaeus vannamei and Oreochromis spp.) with fluorescence detector was developed.The fluoroquinolones residue was extracted by phosphate,purified with n-hexane and solid-phase extraction,quantified using external standard calibration method.The minimum added concentration was 5.0 μg/kg for norfloxacin,ciprofloxacin and enrofloxacin,1.0 μg/kg for danofloxacin,4.0 μg/kg for pefloxacin,20.0 μg/kg for sarafloxacin and 30.0 μg/kg for ofloxacin.The average recoveries of the seven fluoroquinolones in aquatic product ranged from 75% to 95%,with relative standard deviations of 1.3%- 9.3%.The limit of detection (LOD) was 0.07- 5.84 μg/kg, the limit of quantification (LOQ) was 0.23 -19.48 μg/kg.Samples obtained optionally from shanghai terminal market were analyzed with the established method,and no fluoroquinolone was detected.