Identification of an endophytic isolate from Llex cornuta andits antagonism against Rhizoctonia solani

An endophytic isolate (No.2),which has inhibiton on Rhizoctonia solani,was isolated from Ilex cornuta Lindl.et.Paxt.It was identified as Trichoderma harzianum Rifai by morphological and physiological & biochemical characteristics via Biolog Microbial Identification System.The test of dual culture indicated that there were competitive and mycoparasitic behavior by T.harzianum on R.solani.Trichodermin isolated from the broth of T.harzianum showed strong biological activity against R.solani the protective effects and therapeutic effects were 88.89% and 95.83%,respectively,after treatment for 7 days.There was no blight on cucumber seedlings observed after treatment of trichodermin.The results indicated that trichodermin was safe to cucumber plants.