Insecticidal alkaloids from Aconitum carmichaeli produced in Guizhou

The crude and purified total alkaloid quantities of the Aconitum carmichaeli root collected in Guizhou Province were analyzed by the standard protocol in Chinese Pharmacopiea,which showed that the crude total alkaloid 0.928%,and the purified total alkaloid 0.683% are higher in quantity than A.carmichaeli produced in the other places.Three main alkaloids were isolated by solvent extract,silica gel chromatography,thin layer chromatography preparation,recrystallization,and they were identified as hypaconitine,mesaconitine and aconitine by m.p.,ESI-MS,1H and 13C NMR spectral data.The reverse HPLC analysis indicated that the quantities of mesaconitine,hypaconitine,and aconitine in Guizhou Aconitum carmichaeli are 0.20%,0.10% and 0.016%,respectively.