Isolation and identification of antifungal constituents from the leaf of Myrica rubra

Methanol extract of the leaves of Myrica rubra was further partitioned with petroleum ether,chloroform,ethyl acetate and n-butanol respectively and obtained four crude extracts.Antifungal activity of the four crude extracts was tested .It showed that ethyl acetate and n-butanol extract had higher activity.Their structures were identified by ESI-MS,1H NMR and 13C NMR as maslinic acid( 1 ),3-O-trans-p-coumaroyl maslinic acid( 2 ),3-O-galloyl-dihydromyricetin( 3 ),quercetin-7-O-rhamonside( 4 ),myricetin-3-O-rhamnoside( 5 ),myricetin-3-O-rhamnoside(3-O-galloyl)( 6 ),myricetin-3-O- rhamnoside(2-O-galloyl)( 7 ).The inhibition effects of compound 3,4,5 and 6 had been tested by method of spore germination and the result showed that all the 4 compounds have strong inhibiting effect on the tested plant pathogens.Botrytis cinerea and Setosphaeria turcica which LC50 velues were between 3.44-4.59 mg/L and 8.85-11.6 mg/L,respectively.