Development of an enzyme-linked immunosorbent assay for the detection of imidaclothiz

A sensitive indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on polyclonal antibody for detection of imidaclothiz was developed.A hapten of imidaclothiz was synthesized and conjugated with bovine serum albumin (BSA) as the immunogen,conjugated with ovalbumin (OVA) as the coating antigen.Polyclonal antibody was obtained by immunizing New Zealand rabbits with immunogen,and the titer was 2.56×106.The inhibition standard curve of imidaclothiz was obtained under the optimised conditions .The half-maximal inhibition concentration (IC50) and the limit of detection(IC10) were 0.18 mg/L and 0.001 8 mg/L of imidaclothiz,respectively.Very low cross-reactivity was found for some structurally related compounds except imidacloprid.At different spiked levels (0.05-1 mg/kg in water,soil and cabbage) recoveries of imidaclothiz were from 91.42% -113.82%,with rlative sandard deviation(RSD) of 0.72% -8.68%.The ELISA could be a convenient tool for monitoring imidaclothiz residues in environmental and agricultural samples.