Research advances on the resistance of plant pathogenic fungi to SBIs fungicides

The sterol biosynthesis inhibitors (SBIs) killed pathogenic fungi by inhibiting different enzymes activity in biosynthesis pathway of sterols to interfere or block sterol biosynthesis.The recent research progresses of resistance of plant pathogenic fungi to SBIs were summarized,including the resistance status,genetic mechanism,physiological and biochemical mechanism,molecular mechanism and management strategy.According to the results of laboratory studies and field experiments,the resistance of pathogenic fungi against SBIs might cause by one or more than one mechanisms.ABC,MFS transport protein gene and CYP51 protein gene were regarded as the important molecular mechanisms.Among them, ABC transport protein gene was considered as a non-special vehicle to excrete fungicides from inner to outer of cell membrane by flippase enzyme.The over- and background-expression of MFS transport protein gene played key role in the resistance of pathogenic fungi.While point-mutation and over-expression of CYP51 protein gene was easily occurred in plant pathogenic fungi,which decreased the binding affinity of protein to fungicides and thereby resulted in the resistance against SBIs.With the rapid development of molecular biology technology and incorporating with other disciplines,the design of multiple action sites of sterol biosynthesis inhibitors based on the information of resistant related genes,proteins and regulation pathway would be possible,which would then extend the service life of SBIs.