Isolation of the insecticidal ingredients from Periploca sepium

Two compounds,periplocoside D and F were isolated from the methanol extract of the root barks of Periploca sepium by silica gel chromatograph,high pressure liquid chromatograph(HPLC) and bioassay-guided fractionation.The bioassay results showed that periplocoside D and F had stomach toxic activity with the LC50 value of 48 h 0.39 and 0.34 mg/mL against 3rd instar larvae of Mythimna separata,and against 3rd instar larvae of Plutella xyllostella with the LC50 value of 48 h 1.21 and 1.39 mg/mL.