Effects of chlorantraniliprole on expression abundance of ryanodine receptor gene mRNA from Plutella xylostella

The expression abundance of ryanodine receptor(RyR)mRNA from Putella xylostella(L.)larvae treated with chlorantraniliprole,emamectin benzoate and chlorfenapyr respectively were studied by quantitive RT-PCR method.The results showed that chlorantraniliprole could induce the expression of RyR gene from P.xylostella,and emamectin benzoate and chlorfenapyr had no effects on the expression of RyR gene . In order to further identify the induction of chlorantraniliprole to RyR gene,the relative expression abundance of RyR gene from larvae treated continuously and discontinuously with 0.01 mg/L chlorantranilipole were analyzed,and the RyR gene expression abundance from P.xylostella that resistant to chlorantraniliprole was also analyzed.The results suggested that 0.05 mg/L and 0.01 mg/L chalorantraniliprole could induce the expression level of RyR gene from P.xylostella in both treatment;Moreover,the relative expression level of RyR gene from chlorantraniliprole resistant strain was 5.79-fold comparing with that of the susceptible strain.The results would be helpful to indicate the mechanism of chlorantraniliprole,and the results would be possibly to helpful to screen compounds targeting at RyR.