Expression of aminopeptidase N(APN2)from Plutella xylostella larval midgut in Sf9 cells

Using the insect cells-Bac-to-Bac baculovirus expression system to express APN2(aminopeptidase N)protein,the APN2 expression system in Sf9 cells was successfully established.APN2 gene from susceptible and resistant Plutella xylostella(L.)larval midgut were inserted into pFAST Bac HTB vector,expressed in insect cells Spodoptera frugiperda(Sf9)cells,respectively,with Bac-to-Bac baculovirus expression system.A 107 kDa protein was examined and confirmed by SDS-PAGE and Western-blotting.The results showed that P.xylostella larval midgut APN2 gene was successfully expressed in Sf9 cells which provided a foundation for further investigation on function of APN2 and its relationship with Bt resistance.