Preparation and performances of poly(ethyleneglycol)- polycaprolactone micelles loaded with tebuconazole

The poly(ethylene glycol)-polycaprolactone(PEG- b -PCL) block copolymer was synthesized by ring-opening polymerization method,and the self-assembled PEG- b -PCL micelles was applied as carrier to load tebuconazole by self-assembly method.The diameter of the tebuconazole loaded micelles was characterised by dynamic light scattering(DLS) and transmission electron microscope(TEM).The drug loading efficiency,encapsulation rate and release characteristics in vitro were measured by ultraviolet specrophotometer and high performance liquid chromatography(HPLC).The mould inhibition properties of tebuconazole-loaded micelles to Trichoderma viride was evaluated by filter paper method.Results showed that tebuconazole loaded PEG-b-PCL micelles were spherical and uniform,with a mean particle diameter of 250 nm.The drug loading efficiency and encapsulation rate were 26.4% and 18% respectively.Tebuconazole-loaded PEG-b-PCL micelles showed good slow-release characteristics.After 192 h,the drug release percentage of tebuconazole-loaded PEG-b-PCL micelles was 83.35%.Tebuconazole-loaded PEG-b-PCL micelles also exhibited more efficient mould inhibitions to T. viride than that of tebuconazole-ethyl acetate solution.