Study on distribution and metabolism of spirotetramat in rat

A method was developed to determine the residue of spirotetramat and its metabolite in various rat organ by ultra performance liquid chromatograph equipped with triple quadrupole mass spectrometer (UPLC-MS/MS).And the distribution and metabolism of spirotetramat in rat were studied.The spirotetramat and metabolite in rat organs were extracted with methanol,cleaned up by solid phase extraction (SPE),and detected by UPLC-MS/MS.The results showed that it took only 3 minutes for sample analysis by MS,and the limits of quantitation (LOQ) were 0.005 mg/kg.The spirotetramat and metabolite in organs of rat given 250 mg/(kg bw·d) of spirotetramat were analyzed using the method.The test indicated that there were no significant difference of spirotetramat residue among testicle,liver,lung,kidney,heart and plasma and low levels ranged 0.012-0.025 mg/kg.The spirotetramat residues in fat and muscle were lower (P kidney>plasma>lung>heart>testicle>fat>muscle.