Isolation of antifungal protein phosphate-binding protein precursor and identification of its producing strain

Guided by the results of the bioassay against Bacillus cereus,a protein that the antimicrobial activity was not reported in the literature was isolated from the fermentation broth of actinomycete GXWl which was collected from the soil of Guangxi province by ammonium sulfate precipitation,ion-exchange chromatography,gel column chromatography and SDS-PAGE in turn.According to the analysis of protein mass spectroscopy by a BLASTn search in NCBInr database,the antifungal protein was identified as phosphate-binding protein precursor with the molecular weight of 39.18 ku.The strain GXWl was characterized as Streptomyces rubiginosohelvolus based on the morphological characteristics,cultural characteristics,physiological and biochemical characteristics,as well as phylogenetic analysis of 16S rDNA sequence.