Isolation,structure identification and bioactivity of active ingredients from Phryma leptostachya

Two compounds,haedoxane A and E were isolated from the petroleum ether extract of the roots and stems of Phryma leptostachya L.by silica gel chromatograph,high pressure liquid chromatograph(HPLC) and bioassay-guided fractionation.The bioassay results showed that haedoxane A and E had stomach toxic activity with the LC50 value of 24 h 34.9 and 52.8 μg/mL against 3rd instar larvae of Mythimna separata, against 3rd instar larvae of Semiothisa cinerearia with the LC50 value of 24 h 66.1 and 1 675 μg/mL,and against Musca domestica with the LC50 value of 24 h 39.2 and 969 μg/mL.Haedoxane A and E had topical toxic activity with the LD50 value of 0.09 and 0.26 μg/larvae against 3rd instar larvae of M.separata,against 3rd instar larvae of S.cinerearia with the LD50 value of 0.049 and 1.33 μg/larvae,and against M.domestica with the LD50 value of 24 h 0.047 and 1.12 μg/fly.