Determination of pyraclostrobin residues in peanut and peanut hull by gel permeation chromatography-solid phase extraction-high performance liquid chromatography

A method for determination of pyraclostrobin residues in peanut and peanut hull was established.Target pesticide was extracted with acetonitrile,cleaned up with gel permeation chromatography(GPC) and solid phase extraction-amino(SPE-NH2)column,then detected by high performance liquid chromatography-ultraviolet detector(HPLC-UV).Recoveries of the pesticide spiked at 0.05,0.5 and 1 μg/g in peanut were 101.7%,86.5% and 83.2% and in peanut hull were 98.1%,93.8% and 85.3%.The limit of quantification reserched 0.05 mg/kg.